Panel: Total Testosterone
The gold standard testosterone test. Total Testosterone measured by LC/MS-MS (liquid chromatographyโtandem mass spectrometry) and Free Testosterone measured by equilibrium dialysis โ the most accurate methods available.
LC/MS
Mass Spectrometry
ED
Equilibrium Dialysis
3
Biomarkers
Quest
Diagnostics
How It Works
Simple. Accurate. Insurance Billed.
We send your lab order to Quest Diagnostics. Walk into any of 2,000+ Quest locations nationwide โ no appointment needed for most locations.
01
Medical Intake
Complete a brief online intake. A licensed provider reviews your symptoms and orders the appropriate labs.
~5 minutes
02
Visit Quest Lab
Walk into any Quest Diagnostics patient service center. Simple blood draw โ morning fasting recommended.
2,000+ locations
03
Insurance Billed
Your insurance is billed directly by Quest Diagnostics. You're only responsible for your copay/deductible.
Most plans accepted
04
Get Results
Receive your detailed results online. A provider reviews your levels and provides personalized guidance.
3โ5 business days
What's Measured
Total + Free Testosterone โ Gold Standard
This test measures three values using the most accurate methodologies recognized by the Endocrine Society.
| Age 20โ49 | 249โ836 ng/dL |
| Age 50+ | 193โ740 ng/dL |
| Optimal (functional) | 500โ900 ng/dL |
| Age 20โ49 | 9.3โ26.5 pg/mL |
| Age 50+ | 6.8โ21.5 pg/mL |
| % Free Testosterone | 1.0โ3.5% |
Your Report
Your Results โ Clear & Actionable
Results include exact values, reference ranges, and a provider review with personalized guidance.
Simple. Medical. Personalized.
Medical intake
A licensed provider reviews your intake and determines the right test. Your results are reviewed for actionable guidance.
Medical Intake
Answer a few online questions about your health history, lifestyle, and goals โ no clinic visit required.
Personalized plan
Receive a personalized provider review of your testosterone levels with guidance on next steps โ whether that's lifestyle optimization, further testing, or a TRT consultation.
Ongoing support
Stay connected with your care team for follow-ups, adjustments, and expert answers โ all online.
FAQs
Why is LC/MS-MS better than a standard testosterone test?
Standard immunoassays can cross-react with other steroids, producing inaccurate results โ especially at low or high levels. LC/MS-MS physically separates testosterone from other molecules before measuring it, making it the most accurate method available. The Endocrine Society recommends it as the gold standard.
Why is equilibrium dialysis better for Free T?
Most labs offer "calculated" free T (an estimate based on formulas) or "analog" assays (inaccurate). Equilibrium dialysis physically separates free testosterone from protein-bound testosterone using a dialysis membrane โ measuring actual unbound hormone. It's the recognized reference method.
Do I need to fast before this test?
Fasting is not strictly required but is recommended. Testosterone levels are highest in the early morning and can be affected by recent meals. For the most accurate results, draw blood between 7โ10 AM after an overnight fast.
Will my insurance cover this test?
Quest Diagnostics accepts most major insurance plans. The lab bills your insurance directly. You'll be responsible for your copay, deductible, or coinsurance as determined by your plan. If you have symptoms of low testosterone, most plans cover diagnostic testing.
What's the Quest test code?
This test is Quest Diagnostics order code 070038 โ "Testosterone, Free (Dialysis) and Total, MS." It includes Total Testosterone (LC/MS-MS), Free Testosterone (Equilibrium Dialysis), and % Free Testosterone (Calculated).
How long do results take?
Results are typically available within 3โ5 business days after your blood draw. Because equilibrium dialysis is a specialized methodology, it may take slightly longer than standard testosterone immunoassays.
What if my levels are low?
If your Total or Free T is below the reference range, a provider will review your full clinical picture. Options may include lifestyle modifications (sleep, exercise, nutrition, stress management), targeted supplementation, or referral for testosterone replacement therapy (TRT) if medically indicated.
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